摘要：对XX市有代表性蔬菜塑料大棚中不同氮肥用量对土壤的理化性质以及微生物功能多样性的影响进行研究。结果表明，供试土壤为中性偏酸性土壤，在4.99~7.20之间，不同施氮量对土壤EC和pH有显著影响，土壤EC值随着氮肥用量增加增加，而土壤pH值随着氮肥用量增加而降低。习惯氮肥用量土壤水解氮和全氮含量最高，并显著高于其他处理土壤；而不同施氮量对土壤有机质含量影响不显著。土壤微生物AWCD与功能多样性指数包括Shannon多样性指数、Simpson优势度指数和McIntosh均匀度指数均随着氮肥用量的增加显著降低，减氮20%与习惯用量处理显著降低了土壤微生物AWCD与功能多样性指数包括Shannon多样性指数、Simpson优势度指数和McIntosh均匀度指数，减氮40%处理没有明显影响土壤微生物平均吸光值和功能多样性指数包括Shannon多样性指数、Simpson优势度指数和McIntosh均匀度指数。

关键词：设施栽培蔬菜 氮肥用量 微生物功能多样性

Abstract:We investigated soil physi-chemical properties and microbial functional diversity under different nitrogen fertilization in a greenhouse-based intensive vegetable production system in Yixing County, Jiangsu Province. Results showed that the soils were neutral or acidic with PH from 4.99 to 7.20. Nitrogen fertilization rates have significant effects on soil EC and pH. Soil EC was generally increased whilst soil pH was significantly decreased with increase of nitrogen fertilization rates. Soil hydrolyzable nitrogen and total nitrogen contents were signigicantly higher under conventional nitrogen fertilization rate than any other treatment. In contrast, different nitrogen fertilization has little effect on soil total organic carbon. AWCD and the three diversity indices (Shannon index, Simpson index, McIntosh index) were generally decreased with increase of N fertilization rate. These microbial parameters were significantly lower under conventional nitrogen fertilization rate and 80% of conventional nitrogen fertilization rate than under any other treatment. However, 60% of conventional nitrogen fertilization rate did not decrease these microbial parameters significantly. 

Keywords:polytunnel greenhouse vegetable soil; nitrogen fertilization rate; microbial functional diversity.

　　　本实验通过对种植宜兴塑料蔬菜大棚的土壤为研究对象，研究不同施氮量对土壤的理化性质以及微生物功能多样性的影响，借以了解塑料大棚中的土壤肥力状况，为塑料大棚蔬菜土壤可持续利用和合理施肥提供理论依据。