摘要:以生姜粉为原料，通过高效液相色谱（HPLC）测定分配系数法优选高速逆流色谱（HSCCC）分离的最佳溶剂体系，确定了最佳的HSCCC分离条件：溶剂体系为正己烷-乙酸乙酯-甲醇-水（5.5:5:5.5:5，v/v）以及同种体系的（6.5:5:6.5:5，v/v），流速2 mL/min、转速800 r/min、分离温度25℃，经梯度洗脱即可从生姜提取物中分离得到4种单体组分，经HPLC分析为6-姜酚，6-姜烯酚，8-姜酚三种单体成分，纯度较高，还得到只含有8-姜酚和10-姜酚的混合姜酚。

　　　对分离得到的6-姜酚，6-姜烯酚，8-姜酚三种单体成分进行了抗氧化性分析，结果表明具有一定的清除羟基自由基和DPPH自由基能力，清除能力与浓度呈较明显的量效关系；稳定性实验表明，姜酚在非碱性条件下对温度、时间和光照等具有较好的稳定性，适用于食品生产。

关键词 姜酚；高速逆流色谱分离；抗氧化性；稳定性

Abstract:Ginger powder as raw material, by high-performance liquid chromatography (HPLC) determination of the partition coefficient method is preferably high-speed countercurrent chromatography (HSCCC) the separate solvent system to determine the best HSCCC separation conditions: solvent system of n-hexane - ethyl acetate- methanol - water (5.5:5:5.5:5, v/v) and the same kind of system (6.5:5:6.5:5, v/v), flow rate of 2 mL/min, the rotational speed of 800 r/min, separation temperature25 ℃, gradient elution can be separated from the ginger extract monomer component analyzed by HPLC for 6-gingerol, 6-Shogaol 8-ginger phenols three monomer composition, purity than thealso contains only 8-gingerol and 10-gingerol mixed gingerol.

Isolated 6-gingerol, 6-Shogaol, 8-gingerol three monomer components of the antioxidant activity analysis, the results showed that the scavenging DPPH radical scavenging capacity and concentration showed an obvious dose-effect relationship; stability experiments showed that gingerol has good stability under alkaline conditions of temperature, time and light, suitable for food production.

Keywords  gingerol  high-speed counter current chromatography  antioxidant activity  stability