摘要：胶原蛋白（Collagen）是一种细胞外基质的结构蛋白质，在生物科技产业有的极其重要的地位，也是需求量十分庞大的生物医用材料之一。其应用领域包括生物医用材料、化妆品、食品工业等。由于其具有广泛的应用价值，近年来国际上对胶原蛋白的研究日新月异。

　　　本实验主要用丙酮、氯仿、异丙醇等有机溶剂作为脱脂剂。研究了同物料比下的丙酮与异丙醇间的不同体积比的脱脂效果；同物料比下的丙酮与异丙醇间的不同体积比的脱脂效果；同物料比不同脱脂剂同种配比下的脱脂效果。也初步研究了不同的酸（醋酸、柠檬酸）对胶原蛋白的提取效果的影响，对提取物进行了红外、紫外、荧光等的光谱分析，初步确定提取物为胶原蛋白。

关键词：猪皮； 胶原蛋白； 有机脱脂剂；脱脂效率

ABSTRACT：Collagen is a kind of extra cellular matrix structure protein in biological scientific and technological industry some extremely important position, is also extremely huge demand for one of biomedical materials. Its application field, including biomedical materials, the cosmetics, food industry etc. Since it has wideapplication value, in recent years the international research of collagen day.

  This experiment, mainly used acetone, chloroform, isopropanol and other organic solvents as degreasing agents. Study of the same material ratio between acetone and isopropyl alcohol degreasing effect of different volume ratio; compared with the same materials of acetone and isopropanol under different volume ratio between the degreasing effect;compared with same material of different degreasing agents in the same proportion of degreasing effect.Preliminary study of different acids (acetic acid, citric acid) influence on collagen extraction,Of extracts by IR, UV and fluorescence spectra analysis, initially set the extraction of collagen.

Keywords: pigskin;  Collagen;  Organic degreasing agents;  Skimefficiency