摘要：目的：以蟾毒灵为模型药物，聚氰基丙烯酸正丁酯（PBCA）为载体，制备蟾毒灵PBCA纳米粒，并对制备工艺进行筛选。方法：采用乳液聚合法，界面缩聚法，预合成PBCA法，乙醇法等方法制备蟾毒灵PBCA纳米粒。以包封率和载药量为考察指标，选择最佳制备方法，并通过单因素试验优化制备工艺。结果表明：由乙醇法制得的纳米粒，大小较为均匀，平均粒径为149.2nm，多分散系数为0.258,包封率为75.751%，载药量为5.601%。结论：本实验优化的乙醇法制备工艺稳定可行。

关键词：蟾毒灵；α-氰基丙烯酸正丁酯；纳米粒；乙醇法

Abstract  objective：Using bufalin as the model drug, Poly n-butyl cyanoacrylate (PBCA) as the carrier, the bufalin PBCA nanoparticles was prepared and the preparation technology was optimized. Methods: Emulsion polymerization, interfacial polymerization, Pre synthesized PBCA, and ethanol method was used to prepare bufalin PBCA nanoparticles. The encapsulation efficiency and drug loading as investigated. The preparation and formulation was optimaized by single factor experiment. Results :Nanoparticles prepared by anhydrous ethanol has uniform size, the average particle size was 149.2nm, the polydispersity index was 0.258, encapsulation efficiency was 75.751%, drug loading was 5.601%. Conclusion: The optimum ethanol preparation process is stable and feasible.

Keywords:  bufaline; BCA; nanoparticles; ethanol method