摘要:目的 本实验主要研究共生菌有效成分(药物D9)对Poly(I:C)诱导的TNF-α水平的影响。方法 运用流式细胞术检测小鼠巨噬细胞RAW264.7分泌的TNF-α炎症因子水平。分析不同浓度的Poly(I:C)对TNF-α炎症因子浓度的影响；研究药物D9（2μg/ml）对Poly(I:C)（50μg/ml）诱导的TNF-α水平的影响。Cell QuestTM软件分析TNF-α阳性细胞的百分率。结果 不同浓度的Poly(I:C) (50μg/ml, 100μg/ml和200μg/ml) 对TNF-α生成与对照组相比有极显著差异（P<0.001），并且具有浓度梯度依赖性；药物D9能够极显著降低Poly(I:C)诱导的TNF-α的水平（P<0.01）。结论 药物D9可极显著降低Poly( I: C)诱导的RAW264. 7细胞分泌TNF-α的高表达(P<0.01)。

关键词：聚肌苷酸胞苷酸(Poly(I:C))； 共生菌； 肿瘤坏死因子α； 药物D9； 小鼠巨噬细胞RAW264.7

Abstract：Objective To investigate the influence of symbiotic microbes effective component (drug D9) on TNF-α level induced by Poly(I:C). Methods Using flow cytometry RAW264.7 macrophage inflammatory cytokines TNF-α generated. Analysis of different concentrations of Poly(I:C) on the concentration of inflammatory cytokines TNF-α. Study drug D9 (2μg/ml) of Poly(I:C) (50μg/ml) induced TNF-α levels. Cell QuestTM software to analyze the percentage of TNF-α positive cells. Results Different concentrations of Poly(I:C) (50μg/ml, 100μg/ml and 200μg/ml) of TNF-α compared with the control group generated significantly different (P<0.001), and has a concentration gradient-dependent. Drugs D9 Could significantly reduce the Poly(I:C) induced TNF-α levels (P<0.01). Conclusion Drugs D9 can block the Poly(I:C) stimulation of RAW264. 7 cells secreted high expression of TNF-α (P<0.01).Key words： Poly(I:C)； symbiotic microbes； TNF-α； drug D9； RAW264.7