需要金币： 1000 个金币 |
资料包括：完整论文 |  |
 |
| 转换比率：金额 X 10=金币数量， 例100元=1000金币 | 论文字数：5216 |  | |
| 折扣与优惠：团购最低可5折优惠 - 了解详情 | 论文格式：Word格式(*.doc) |  |
摘 要:目的：从转基因大豆中提取DNA并对大豆中转基因成分进行检测。方法：CTAB法、PCR扩增技术、设计并合成引物对转基因大豆的内源基因Lectin；外源基因CaMV35S 启动子、NOS终止子和Cp4 EPSPS基因进行检测。结果：转基因大豆中含有内源基因Lectin；外源基因CaMV35S 启动子、NOS终止子和Cp4 EPSPS基因。 关键词:转基因大豆；转基因成分定性PCR检测；Lectin基因；CaMV35S基因；Cp4 EPSPS基因
Abstract：Objective: To extract DNA from genetically modified soybean and to detect Genetically Modified Components in Soybean. Methods: DNA was extracted from soybean by CTAB method, the technique of PCR amplification, The primers for endogenous gene Lectin of genetically modified soybean, CaMV35S promoter gene , NOS terminator and Cp4 epsps gene were designed and synthesized. Results: There are endogenous gene Lectin , CaMV35S promoter gene , NOS terminator and Cp4 epsps gene in genetically modified soybean. Key words：genetically modified soybean; qualitative PCR detection of genetically modified organisms; Lectin gene; CaMV35Sgene; Cp4 EPSPS gene. |
